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c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. The JNK Activity screening kit utilizes an N-terminal c-Jun (1-79) fusion protein bound to glutathion sepharose beads to selectively “pull down” JNK from cell lysates. After wash to remove nonspecifically bound proteins, the kinase reaction is then carried out in the presence of cold ATP. c-Jun phosphorylation is then measured by Western blot analysis using a phospho-c-Jun specific antibody.
• Simple procedure • The kit is suitable for both research and drug discovery • The assay utilizes a N-terminal c-Jun fusion protein bead to selectively “pull down” JNK from cell lysate c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody.
• Detection method: Western blot analysis with anti:Phospho:cJun (Ser 73) Specific Antibody at 1:1000 dilutions • Species reactivity: Mammalian • Applications: The kit can be used to screen JNK activity relatively quickly for large numbers of samples.
• Kinase Extraction Buffer • c-Jun (1-79) Fusion Protein Beads • Kinase Assay Buffer • ATP (10 mM) • Phospho-cJun Specific Antibody
Cell and tissue lysates