Description

Size

96 Tests

Catalog no

EKU09897

Quick order process

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Details

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Creatine Kinase, Brain were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Creatine Kinase, Brain were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Test Principle

No significant cross-reactivity or interference between Creatine Kinase, Brain and analogues was observed.

Cross Activity

No significant cross-reactivity or interference between Creatine Kinase, Brain and analogues was observed.

Specificity

This assay has high sensitivity and excellent specificity for detection of Creatine Kinase, Brain

Research Area

Enzyme & Kinase, Metabolic pathway, Cardiovascular biology, Neuro science

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Shelf life

Use Human Creatine Kinase, Brain (CKB) ELISA Kit (CLIA) before 8 months

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Sample Type

Tissue homogenates, Cell lysates and other biological fluids.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Application

For research use only. Not for diagnostic procedures.

Storage Temperature

Short term: 4°C; Long term: see manual.

Assay Type

Double-antibody Sandwich

Target's alterntive name

CK-BB, CKBB, CK-B, CK-1

Target Name

Creatine Kinase, Brain

Species Reactivity

Homo sapiens (Human)

Estimated Turnaround Time

6-11 Business days

Assay Time

2 hours, 40minutes

Detection Method

Chemiluminescent

Detection Range

0.27-200 ng/mL

Sensitivity

0.11 ng/mL

Shipping Conditions

Ice packs

Tissue

brain