Description

Size

96 Tests

Catalog no

EKU03492

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Details

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Creatine Kinase, Muscle were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Creatine Kinase, Muscle were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Test Principle

No significant cross-reactivity or interference between Creatine Kinase, Muscle and analogues was observed.

Cross Activity

No significant cross-reactivity or interference between Creatine Kinase, Muscle and analogues was observed.

Sample Type

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Creatine Kinase, Muscle

Target's alterntive name

CK-MM, CKMM, CK3, CK-M, MM-CK, M-CK, Creatine kinase M-type, N-terminally processed

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Shelf life

Use Human Creatine Kinase, Muscle (CKM) ELISA Kit before 8 months

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Application

For research use only. Not for diagnostic procedures.

Storage Temperature

Short term: 4°C; Long term: see manual.

Research Area

Enzyme & Kinase, Cardiovascular biology

Assay Type

Double-antibody Sandwich

Target Name

Creatine Kinase, Muscle

Species Reactivity

Homo sapiens (Human)

Estimated Turnaround Time

6-11 Business days

Detection Range

1.56-100 ng/mL

Detection Method

Colorimetric

Sensitivity

0.61 ng/mL

Shipping Conditions

Ice packs

Assay Time

3 hours

Tissue

muscle