Description
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Details

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.

Antigen background

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic peptide derived from human Creatine Kinase MM

Synonyms

CKMM; M-CK; Creatine kinase M-type; Creatine kinase M chain; CKM

Specificity

This is a highly specific antibody against Creatine Kinase MM.

Long name

Creatine Kinase MM Polyclonal Antibody, FITC Conjugated

Purification method

This antibody was purified via Protein A.

Host Organism

Rabbit (Oryctolagus cuniculus)

Category

Conjugated Primary Antibodies

Clone

Polyclonal antibody

Recommended dilutions

IF(IHC-P)(1:50-200)

Target Antigen

Creatine Kinase MM

Additional conjugation

Anti-FITC Antibody

Crossreactivity

Human, Mouse, Rat

Immunogen_range

246-266/381

Excitation Emission

494nm/518nm

Concentration

1ug per 1ul

Clonality

Polyclonal

Modification

Unmodified

French translation

anticorps

Tested applications

IF(IHC-P)

Subcellular location

Helical

Swiss Prot

P06732

Modification site

None

Conjugation

FITC

Gene ID number

1158