Description
Quick order process

We invite you to purchase this product. If you have any questions, please contact us. Our specialists will certainly advise the best solution.

Details

Description

A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

Assay principle

The microplate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Brain (CKB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Creatine Kinase, Brain (CKB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Creatine Kinase, Brain (CKB) level in the sample or standard.;

ELISA's stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Precision of the test

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Creatine Kinase, Brain (CKB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Creatine Kinase, Brain (CKB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV

Storage recommendation

-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

ELISA's specificity

This assay has high sensitivity and excellent specificity for detection of Creatine Kinase, Brain (CKB). No significant cross-reactivity or interference between Creatine Kinase, Brain (CKB) and analogues was observed.

Warnings

Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

ELISA's cross-reactivity

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

QC

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Samples to be analyzed

Tissue homogenates, cell lysates and other biological fluids.

Notes

For research use only. Not for diagnostic procedures.

Protocol

Please see ELISA's datasheet, otherwise contact us

Alternate protein number

Please refer to SwissProt

Alternate gene name

CK-BB; CKBB; CK-B; CK-1

Gene name

Creatine Kinase, Brain

Verified reactivity

Homo sapiens (Human)

Estimated production time

7-11 business days

ELISA detection

Chemiluminescent

Detection limits

3.12-200 ng/mL

Assay duration

2 hours, 40min

Research main area

Neuro science;

Sensitivity limit

0,11 ng/mL

Use for

8 months

Shipping requirements

Blue ice

Assay class

Sandwich

Protein number

P12277

Tissue

brain

Verified applications

CLIA

Gene number

1152