Summary of the prodecure

1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.

Tips

Due to transportation and handling small amounts of the liquid components of the Cow Creatine kinase B-type ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.

Kit contents

ELISA test kit for Bovine Creatine kinase B-typeAssay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual

Shipping, storage, shelf life

The Creatine kinase B-type ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Bovine Creatine kinase B-type ELISA Kit is 6 months after delivery.

General description

This is a ready to use kit for performing ELISA tests for detection of Creatine kinase B-type in samples from Bovine

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Sample types

Our Creatine kinase B-type ELISA kit has been proven to work and successfully detect Creatine kinase B-type in Cow

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Long name

ELISA test kit for Bovine Creatine kinase B-type

Synonym name

Cow Creatine kinase B-type ELISA Kit

Applications

Enzyme Linked Immunosorbent Assay

Antigen

Creatine kinase B-type

Species reactivity

Bovine

Detection wavelengt

450 nm

Assay procedure

1-4h

Bovine Creatine kinase B-type ELISA Kit

Size

Catalog no