About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Usage

Western blot (WB) analysis of Creatine Kinase M (Y14) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:NIH-3T3 cell lysate Lane3:H9C2 cell lysate

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Specificity

Creatine Kinase M (Y14) polyclonal antibody detects endogenous levels of Creatine Kinase M protein.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Immunogen

Synthetic peptide, corresponding to the N-terminus of Human Creatine Kinase M.

Alternative Name

M-CK; CKMM; Creatine kinase M-type; Creatine kinase M chain; CKM; CK-M; MCK

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Note

For research use only, not for use in diagnostic procedure.

Application_all

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Group

Polyclonals and antibodies

Reactivity

Human,Mouse,Rat

Applications

WB IHC IF

French translation

anticorps

BiowMW

~ 43 kDa

Swiss-Prot

P06732

Creatine Kinase M (Y14) polyclonal antibody

Size

Catalog no