Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Phosphocreatine (PCr) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Phosphocreatine (PCr) and unlabeled Phosphocreatine (PCr) (Standards or samples) with the pre-coated antibody specific to Phosphocreatine (PCr). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Phosphocreatine (PCr) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Phosphocreatine (PCr) in the sample.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.

Alternative Names

CP; Creatine Phosphate; Phosphorylcreatine; Creatine-P; Phosphagen; Fosfocreatine

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Group

PCR, polymerase chain reaction

Assay Type

Competitive Inhibition

Detection range

123.5-10000ng/mL

Standard

10000ng/mL

Sensitivity

49.3ng/mL

General PCr(Phosphocreatine) ELISA Kit

Size

Catalog no