Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to CKMM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CKBB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CKMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CKMB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

CK2; CK-MB; Creatine Kinase,Muscle/Brain

Research Area

Enzyme & Kinase;Cardiovascular biology;

Latin name

Rattus norvegicus

Detection range

1.56-100ng/mL

Sensitivity

0.67ng/mL

Assay Type

Sandwich

Standard

100ng/mL

Rat CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit

Size

Catalog no