96 Tests

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Test Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit CKMB . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit CKMB. The concentration of Rabbit CKMB in samples can be calculated by comparing the OD of the samples to the standard curve.


Rabbits are used for polyclonal antibody production by Biomatik. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biomatik adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.


E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Shelf life

Use Rabbit CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit before 6 months

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Cross Activity

No significant cross-reactivity or interference was observed.


ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.


For research use only. Not for diagnostic procedures.

Research Area

Cardiovascular, Metabolism, Signal transduction

Storage Temperature

Short term: 4°C; Long term: see manual.

Sample Type

Serum, Plasma, Biological Fluids

Latin name

Oryctolagus cuniculus

Estimated Turnaround Time

6-11 business days

Detection Range

0.16-10 ng/mL

Detection Method


Shipping Conditions

Ice packs

Assay Time

4.5 hours


0.1 ng/mL

Assay Type


Species Reactivity


Target's alterntive name


Target Name