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This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CKM . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CKM and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CKM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CKM. The concentration of Rat CKM in samples can be calculated by comparing the OD of the samples to the standard curve.
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Use Rat CKM (Creatine Kinase, Muscle) ELISA Kit before 6 months
No significant cross-reactivity or interference was observed.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
The kit is manufactured at ISO 9001 certified facilities.
For research use only. Not for diagnostic procedures.
Cardiovascular, Metabolism, Signal transduction
Short term: 4°C; Long term: see manual.
Serum, Plasma, Biological Fluids
6-11 business days