Properties

If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Additional description

Monoclonals are usually produced in mouse as host species and polyclonals in rabbit or goat. Multi species reactivity is achieved by selecting consensus epitopes when blasting the gene for a reactive, innugenic epitope that is the same in the different target species.

Specifications

Host: Rabbit; Species Reactivity: Pan-species; Clonality: polyclonal; Tested applications: WB; IHC; ICC; IP.; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC

Additional_information

Sequence of the immunogen: Inquire for antigen sequence.; Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Storage_and_shipping

Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.

Description

A Rabbit polyclonal antibody against Pan-species Phosphocreatine (PCr). This antibody is labeled with FITC.

Group

PCR, polymerase chain reaction,Polyclonals and antibodies

Notes

Research Use Only.

Conjugation

Anti-FITC Antibody

French translation

anticorps

Phosphocreatine (PCr) Polyclonal Antibody (Pan-species), FITC

Size

Catalog no