Gene

Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein. OVA is also the best characterized and the first antigen proteins used as a transgene to make transgenic mice. Many different transgenic mouse models have systemic OVA expression driven by the ubiquitously expressed b-actin promoter or tissue-specific OVA expression with insulin promoter to drive the transgene expression, for studying type I diabetes, or in different isoforms, secreted or cell-membrane associated, and more recently as inducible transgene models. These C57BL/6 mice, BALB/c mice models are well characterized, and have contributed to our understanding of immunogenicity and tolerance by the OVA model.

About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.

Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

Alternative Names

CP; Creatine Phosphate; Phosphorylcreatine; Creatine-P; Phosphagen; Fosfocreatine

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Group

PCR, polymerase chain reaction

Application

Immunogen; SDS-PAGE; WB.

Organism species

Pan-species (General)

Expression System

Protein conjugation

Source

Protein conjugation

Traits

Freeze-dried powder

Tag

N-terminal His Tag

Item Name

Phosphocreatine

Predicted Molecular Mass(KD)

211.1g/mol

Isoelectric Point

C4H10N3O5P

Purity

> 95%

OVA Conjugated Phosphocreatine (PCr)

Size

Catalog no